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NaM702001_Lolina Bst Plus DNA Polymerase-Lolina

NaM702001_Lolina Bst Plus DNA Polymerase

    詳細(xì)說(shuō)明

    Lolina A/S

    Address: Sindalsvej 30 8240 Risskov Danmark 

                        Email: Info@lolina.dk

     Website: https://lolina.dk



    Product Specification

     

    Product

    Lolina? Bst Plus DNA Polymerase (40 U/μL)

    Catalog No.

    NaM702001

    Storage conditions

    Store at -25 ~ -15℃, valid for 2 year.

     

    Enzyme Storage Buffer

    10 mM Tris-HCl,   50 mM KCl, 0. 1 mM EDTA, 1 mM DTT, 0. 1% Triton X-100, 50% Glycerol pH 7.5 @ 25°C

    Heat Inactivation

    85°C, 5 min

     

    Unit Definition

    1 unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitate under the reaction conditions of 65°C for 30 minutes.

     

    Product Applications

    This product is suitable   for various isothermal amplification reactions such as LAMP, CPA, RCA, etc.

     

     

    Product description

     

    Lolina?  Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I,

    lacking 5 ′-3′ exonuclease activity. The enzyme has stronger 5 ′-3′ DNA polymerase activity, strand displacement  activity  and   dUTP  tolerance,  which   is  more   suitable  for  anti-pollution  isothermal amplification reactions, such as LAMP, CPA, etc.

     

    CAT

    Components Name

    Size

    8,000 U

    40,000 U

    NaM702001-A

    Lolina? Bst Plus DNA   Polymerase (40 U/μL)

    200 μL

    1 mL

    NaM702001-B

    10× Lolina? Bst Plus DNA Polymerase   Buffer

    1 mL

    3 × 1 mL

    NaM702001-C

    100 mM MgSO4

    1 mL

    3 × 1 mL

     

    Instructions

     

    1.Reaction System


    Components

    Volume (μL)

    Final Concentration

    10x Reaction Buffer

    2.5

    1 x

    100 mmol/L MgSO4

    0.75

    3 mmol/L+ 2 mmol/L in buffer =

    5 mmol/L

    dNTP Mix (25 mmol/L each)

    1.4

    1.4 mmol/L each

    dUTP (25 mmol/L) (optional)

    1.4

    1.4 mmol/L

    UDGase (1U/μl) (optional)

    1

    0.04 U

    DNA

    10 ng – 1μg

    -

    10x Primers

    2.5

    -

    Lolina? Bst Plus DNA Polymerase (40 U/μl)

    1

    1.6 U/μl

    ddH2O

    to 25



    [Note]:

    a)   The amount of  Lolina?  Bst Plus DNA Polymerase can be adjusted and optimized according to different experiments.

    b)   10× Reaction Buffer: 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Tween-20, pH 8.8 @ 25°C.

         c)   The Mg2+  concentration can be adjusted between 4 – 10 mM according to different           experiments.

        d)   10× Primers: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B each.

     


    2.Reaction  ro  ram


     

    Temp.

    Time

    Function

    25 - 37°C

    5 – 10 min

    Degradation of templates containing uracil (optional)

    65°C

    30 – 60 min

    Reaction

    85°C

    5 min

    Inactivation

     

    Notes

    1.         The enzyme should be stored in a cooler or on ice during use and immediately placed at -20°C after use. This product is for research use only.

    2.         For your safety and health, please wear a lab coat and disposable gloves during operation.



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